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This made sure we would have comparable mathematical electricity while using the brand new RNA-Seq otherwise Ribo-Seq studies

This made sure we would have comparable mathematical electricity while using the brand new RNA-Seq otherwise Ribo-Seq studies

The identification of genes that are significantly up-regulated or down-regulated using RNA-Seq and Ribo-Seq data can be used to differentiate between genes that are likely to be regulated at the level of transcription (both RNA-Seq and Ribo-Seq show the same tendency) from those that are regulated primarily at the level of translation (significantly up-regulated or down-regulated by Ribo-Seq but not RNA-Seq data), or that undergo post-transcriptional buffering of gene expression (only significant by RNA-Seq) . In order to perform differential gene expressoin (DGE) analysis for each experiment we subsampled the CDS table of counts so as to have approximately the same number of mapped reads in each of the samples.